The Geneaid™ Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA is bound to the column and contaminants are efficiently removed. The purified plasmid DNA is eluted then precipitated with isopropanol for desalting. The entire procedure can be completed without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.
Advantages
- High Yield: >400 µg ofpure transfection grade plasmid DNA from 100 ml of cultured bacterial cells
- Sample Volume: 50-300 ml of cultured bacterial cells
- Format: plasmid midi anion-exchange resin column
- Elution Volume: 500 µl - 2 ml
- Operation time: within 2 hours (only 80 minutes when combined with the Presto™ Plasmid Concentrator)
- Storage: dry at room temperature (15-25ºC) for up to 2 years without showing any reduction in performance
Applications
Transfection, Sequencing, Ligation, PCR, In-vitro Transcription, Microinjection, Restriction Enzyme Digestion, Gene Gun
Components
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PM1, PM2, PM3 Buffer
-
TrueBlue Lysis Buffer
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PEQ Buffer
-
PW Buffer
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PEL Buffer
-
RNase A
- Plasmid Midi Columns
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